Hannah Hampton will miss England's friendly against Brazil on Saturday because of an elbow injury, meaning there will be a debut for one of the other goalkeepers in the squad.
The 24-year-old was one of the stars of England's Euro 2025 victory, saving two penalties in the final shootout win against Spain.
One of the back-up goalkeepers, Khiara Keating or Anna Moorhouse, is expected to feature at Etihad Stadium in Hampton's absence, though manager Sarina Wiegman would not reveal who.
Manchester City goalkeeper Keating, 21, and Orlando Pride's Moorhouse, 30, were both part of the England squad at Euro 2025 but did not feature, while Brighton's Sophie Baggaley has been called up to the squad this week to replace Hampton.
Arsenal defender Lotte Wubben-Moy (illness) and Manchester United midfielder Jess Park (concussion) will also miss Saturday's game.
"Of course it's not nice to have an injury but it's not a major injury. It's also an opportunity for the other goalkeepers," Wiegman told BBC Radio 5 Live.
"These friendly games are good to try out things and we were going to do that anyway. With goalkeepers it's always harder as there's just one position.
"They are two very different goalkeepers in Khiara Keating and Anna Moorhouse. Khiara is a young, very talented player.
"Khiara is very explosive on the line and has good skills playing out from the back. Anna is tall so good at defending crosses. At her club, they play a little differently and are more direct, so they are the differences."
The Lionesses are playing four successive friendlies as part of a homecoming series to end the year, celebrating their European success along the way.
England defender Alex Greenwood praised the influence of the other goalkeepers during Euro 2025, telling BBC Radio 5 Live: "They have been super patient. Over the summer they did a fantastic job of helping Hannah - and the team as well.
"The keepers stayed out after training for a long time to do extra shooting practise and penalty shootouts. Sometimes their roles go unnoticed."
