Harry Maguire, Kyle Walker and Sam Johnstone have left England's camp due to injuries.
James Trafford and Rico Lewis have moved across from the under-21 squad to replace them.
It is Burnley goalkeeper Trafford’s first senior call up.
Manchester City’s Lewis has one senior cap having made his debut for Gareth Southgate’s side in North Macedonia in November 2023.
Maguire, Walker and Johnstone have returned to their respective clubs for assessment having sustained injuries in recent days.
England play Belgium at Wembley Stadium on Tuesday.
