Preston-born Lennyjohnrose had battled motorneuronedisease and went public with it in 2017 in a bid to raise awareness of the disease and also funds for his charity.
Announcing the news on its facebook page burnleyfootballclub said: "Our thoughts are with Len's family and friends at this very sad time. "You'll always be a Claret Len."
Johnrose began his career at Blackburn Rovers as a young trainee, and also spent time on loan at Preston North End.
During these spells he impressed to a level where he was signed for £50,000 by Hartlepool United. He went on to make over 50 appearances for the team, scoring 11 goals in the process.
At the end of his contract, he was signed by Bury manager Stan Ternent who wanted to add some toughness to his central midfield.
Johnrose was a central character in Bury's rapid rise to the First Division. Stan Ternent saw the midfielder as so important to his sides that he paid £225,000 to take Johnrose with him to his new club, Burnley.
At the end of the 2002-03 season he was released on a free transfer, but re-signed for the club four months later on a week-to-week basis. Later he re-signed for another of his old clubs, Bury. After three months he again moved, this time to Swansea City.
Johnrose helped Swansea City avoid relegation from the Third Division out of the Football League, scoring three crucial goals that season.
After retiring, Johnrose became a teacher.
